COL5A1 Monoklonaler Antikörper

COL5A1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C2C2

Kat-Nr. : 67604-1-PBS

Synonyme

2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V

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  WB analysis of HT-1080 using 67604-1-Ig (same clone as 67604-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 67604-1-Ig (same clone as 67604-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67604-1-Ig (same clone as 67604-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67604-1-Ig (same clone as 67604-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody (67604-1-Ig, Clone: 2C2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67604-1-PBS bindet in WB, IHC, IF/ICC, ELISA COL5A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: native protein
• Vollständiger Name: collagen, type V, alpha 1
• Berechnetes Molekulargewicht: 184 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: NM_000093
• Gene symbol: COL5A1
• Gene ID (NCBI): 1289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.