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COL8A1 Polyklonaler Antikörper

COL8A1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17251-1-PBS

Synonyme

COL8, C3orf7, Collagen alpha-1(VIII) chain, Collagen Type VIII

Filter:
  • Western Blot (WB) analysis of various lysates using COL8A1 Polyclonal antibody (17251-1-AP)

    WB analysis using 17251-1-AP (same clone as 17251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using COL8A1 Polyclonal antibody (17251-1-AP)

    IP experiment of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    IP result of anti-Collagen Type VIII (IP:17251-1-AP, 4ug; Detection:17251-1-AP 1:500) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COL8A1 Polyclonal antibody (17251-1-AP)

    IF Staining of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COL8A1 antibody (17251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COL8A1 Polyclonal antibody (17251-1-AP)

    IF Staining of HepG2 using 17251-1-AP (same clone as 17251-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COL8A1 antibody (17251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17251-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17251-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA COL8A1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen COL8A1 fusion protein Ag10607
Vollständiger Name collagen, type VIII, alpha 1
Berechnetes Molekulargewicht 744 aa, 73 kDa
Beobachtetes Molekulargewicht 73 kDa
GenBank-ZugangsnummerBC013581
Gene symbol COL8A1
Gene ID (NCBI) 1295
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.