Collagen Type XI Polyklonaler Antikörper

Collagen Type XI Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 21841-1-AP

Synonyme

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

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  WB analysis using 21841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 21841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

21841-1-AP bindet in WB, IHC, IF-P, ELISA Collagen Type XI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type XI fusion protein Ag16509
• Vollständiger Name: collagen, type XI, alpha 1
• Berechnetes Molekulargewicht: 1818 aa, 182 kDa
• Beobachtetes Molekulargewicht: 250 kDa, 181 kDa
• GenBank-Zugangsnummer: BC117697
• Gene symbol: COL11A1
• Gene ID (NCBI): 1301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)

Publikationen

Species	Application	Title
human	IF	Transl Oncol; Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer; Authors - Nanxin Zheng
human	IHC	J Orthop Res; Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration; Authors - Yusuke Inoue
human	IHC,IF	BMC Med Genomics; Analysis of cancer-associated fibroblasts related genes identifies COL11A1 associated with lung adenocarcinoma prognosis; Authors - Haosheng Zheng
human	IF	Cancer Res; Dissecting FAP+ Cell Diversity in Pancreatic Cancer Uncovers an Interferon-Response Subtype of Cancer-Associated Fibroblasts with Tumor-Restraining Properties; Authors - Joshua Cumming
human	IHC	Oral Oncol; Single-cell transcriptomics reveal the effects of chemoimmunotherapy on hypopharyngeal cancer and its tumor microenvironment; Authors - Xiaoli Zhu
human	IHC,IF	Oncogene; Single-cell landscape of undifferentiated pleomorphic sarcoma; Authors - Yifei Lu