Collagen Type XI Polyklonaler Antikörper

Collagen Type XI Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21841-1-PBS

Synonyme

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

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  WB analysis using 21841-1-AP (same clone as 21841-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21841-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Collagen Type XI und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type XI fusion protein Ag16509
• Vollständiger Name: collagen, type XI, alpha 1
• Berechnetes Molekulargewicht: 1818 aa, 182 kDa
• Beobachtetes Molekulargewicht: 250 kDa, 181 kDa
• GenBank-Zugangsnummer: BC117697
• Gene symbol: COL11A1
• Gene ID (NCBI): 1301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)