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Collagen Type XXV Polyklonaler Antikörper

Collagen Type XXV Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12738-1-PBS

Synonyme

COL25, COL25A1, Alzheimer disease amyloid-associated protein, CLAC-P, COL 25A1

Filter:
  • Western Blot (WB) analysis of various lysates using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    WB analysis using 12738-1-AP (same clone as 12738-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12738-1-AP (Collagen Type XXV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    IF Staining of HeLa using 12738-1-AP (same clone as 12738-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using Collagen Type XXV Polyclonal antibody (12738-1-AP)

    IF Staining of Neuro-2a using 12738-1-AP (same clone as 12738-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12738-1-PBS bindet in WB, IF/ICC, Indirect ELISA Collagen Type XXV und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Collagen Type XXV fusion protein Ag3634
Vollständiger Name collagen, type XXV, alpha 1
Berechnetes Molekulargewicht65 kDa
Beobachtetes Molekulargewicht55 kDa
GenBank-ZugangsnummerBC036669
Gene symbol COL25A1
Gene ID (NCBI) 84570
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Collagen alpha-1(XXV) chain is a protein that in humans is encoded by the COL25A1 gene. COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease brains.(PMID: 11927537)