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Collagen Type XXVII Polyklonaler Antikörper

Collagen Type XXVII Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15673-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Collagen Type XXVII Polyclonal antibody (15673-1-AP)

    WB analysis using 15673-1-AP (same clone as 15673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15673-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Collagen Type XXVII Polyclonal antibody (15673-1-AP)

    IHC staining of human placenta using 15673-1-AP (same clone as 15673-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15673-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15673-1-PBS bindet in WB, IHC, Indirect ELISA Collagen Type XXVII und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Collagen Type XXVII fusion protein Ag8140
Vollständiger Name collagen, type XXVII, alpha 1
Berechnetes Molekulargewicht 1860 aa, 87 kDa
Beobachtetes Molekulargewicht68-73 kDa
GenBank-ZugangsnummerBC007696
Gene symbol COL27A1
Gene ID (NCBI) 85301
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Collagen Type XXVII, also named COL27A1, it is a member of the collagen fibrillar gene family . It is located in the extrcellular matrix. The calculated molecular weight of COL27A1 is 186 kDa. This antibody can recognize the 73 kDa isoform of target. The high level of Col27a1 expression in mouse cartilage suggested the possibility that Col27a1 may be regulated in cartilage by SOX9, as are Col2a1, Col11a2, Col9a1, and Col9a2 (PMID: 15922909). Expression analyses of the mouse col27a1 gene demonstrated high expression in cartilage, the eye and ear, but also in lung and colon. It is likely that the major protein product of COL27A1, proa1(XXVII), is a component of the extracellular matrices of cartilage and these other tissues. Study of this collagen should yield insights into normal chondrogenesis, and provide clues to the pathogenesis of some chondrodysplasias and disorders of other tissues in which this gene is expressed (PMID: 12714037).