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Collagen Type XXVIII Polyklonaler Antikörper

Collagen Type XXVIII Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24401-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Collagen Type XXVIII Polyclonal antibody (24401-1-AP)

    WB analysis using 24401-1-AP (same clone as 24401-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24401-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Collagen Type XXVIII Polyclonal antibody (24401-1-AP)

    IF Staining of U2OS using 24401-1-AP (same clone as 24401-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24401-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24401-1-PBS bindet in WB, IF/ICC, Indirect ELISA Collagen Type XXVIII und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Collagen Type XXVIII fusion protein Ag21459
Vollständiger Name collagen, type XXVIII, alpha 1
Berechnetes Molekulargewicht 1125 aa, 117 kDa
Beobachtetes Molekulargewicht72 kDa, 100-117 kDa
GenBank-ZugangsnummerBC136892
Gene symbol COL28A1
Gene ID (NCBI) 340267
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL28A1 (collagen type XXVIII alpha 1 chain), also known as COL28. It is expected to be located in the basement membranes, cytoplasm and nucleus. And the protein is mainly expressed in salivary gland and adrenal. This antibody can recognize the 117 kDa and 72 kDa isoforms of target. COL28A1 is a new collagen that cannot clearly be assigned to any collagen subgroup, despite the fact that it contains VWA domains. Ongoing investigations will shed further light on the role of COL28A1 in the assembly of nerve fiber basement membranes. Although no human mutation has been associated with COL28A1, it is tempting to speculate that mutations in this gene would lead to neurodegenerative disease (PMID: 16330543).