Complement factor B Polyklonaler Antikörper

Complement factor B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10170-1-PBS

Synonyme

CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD

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  WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  WB analysis of human blood using 10170-1-AP (same clone as 10170-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 10170-1-AP (same clone as 10170-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10170-1-AP (same clone as 10170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10170-1-AP (same clone as 10170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10170-1-PBS bindet in WB, IHC, Indirect ELISA Complement factor B und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Complement factor B fusion protein Ag0226
• Vollständiger Name: complement factor B
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 93-100 kDa, 60-69 kDa
• GenBank-Zugangsnummer: BC007990
• Gene symbol: Complement factor B
• Gene ID (NCBI): 629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

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