Complement factor B Rekombinanter Antikörper

Complement factor B Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241141A8

Kat-Nr. : 84101-5-PBS

Synonyme

CFB, CFAB, C3/C5 convertase, BFD, BF

---

• 

  WB analysis using 84101-5-RR (same clone as 84101-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84101-5-RR (Complement factor B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84101-5-RR (same clone as 84101-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor B antibody (84101-5-RR, Clone: 241141A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84101-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84101-5-RR against Human Complement factor B were performed. The affinity constant is 2.27 nM.

---

Geprüfte Anwendungen

Produktinformation

84101-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Complement factor B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Complement factor B fusion protein Ag0226
• Vollständiger Name: complement factor B
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 93-100 kDa
• GenBank-Zugangsnummer: BC007990
• Gene symbol: Complement factor B
• Gene ID (NCBI): 629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease that is associated with C3b and forms the alternative pathway for C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.