Complement factor D Rekombinanter Antikörper

Complement factor D Rekombinant Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242244C2

Kat-Nr. : 84851-4-PBS

Synonyme

CFD, 242244C2, ADIPSIN, C3 convertase activator, complement factor D (adipsin)

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  WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84851-4-RR (Complement factor D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01660-3

  Sandwich ELISA standard curve of MP01660-3, Human Complement factor D Recombinant Matched Antibody Pair - PBS only. 84851-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1189. 84851-4-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL.

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  Sample test of MP01660-3

  Serum of eight individual healthy human donors was measured. The Complement factor D concentration of detected samples was determined to be 838.5 ng/mL with a range of 561.9-1525.4 ng/mL.

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  IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 84851-4-RR (same clone as 84851-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Complement factor D antibody (84851-4-RR, Clone: 242244C2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84851-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84851-4-RR against Human Complement factor D were performed. The affinity constant is 60.2 pM.

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Geprüfte Anwendungen

Produktinformation

84851-4-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Complement factor D und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Complement factor D fusion protein Eg1189
• Vollständiger Name: complement factor D (adipsin)
• Berechnetes Molekulargewicht: 27kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: NM_001928.3
• Gene symbol: CFD
• Gene ID (NCBI): 1675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).