Connexin 43 Rekombinanter Antikörper

Connexin 43 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

3O6

Kat-Nr. : 80543-1-RR

Synonyme

Connexin-43, GJA1, 3O6, CX43, Gap junction alpha 1 protein

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  WB analysis using 80543-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 80543-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 80543-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 80543-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 80543-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 80543-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 80543-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 80543-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80543-1-RR against Human Connexin 43 were performed. The affinity constant is 5.19 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80543-1-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA Connexin 43 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Connexin 43 fusion protein Ag25533
• Vollständiger Name: gap junction protein, alpha 1, 43kDa
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC026329
• Gene symbol: Connexin-43
• Gene ID (NCBI): 2697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).