Cortactin Rekombinanter Antikörper

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Cortactin Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241987A12

Kat-Nr. : 84569-1-PBS

Synonyme

CTTN, SRC8, EMS1, 241987A12

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  WB analysis using 84569-1-RR (same clone as 84569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84569-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 84569-1-RR (same clone as 84569-1-PBS)

  WB result of Cortactin antibody (84569-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and Cortactin knockout HAP1 cells. This data was developed using the same antibody clone with 84569-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01412-2

  Sandwich ELISA standard curve of MP01412-2, Human Cortactin Recombinant Matched Antibody Pair - PBS only. 84569-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1940. 84569-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01412-2

  The mean Cortactin concentration was determined to be 848.3 ng/mL in A549 cell extract based on a 2.3 mg/mL extract load, 581.1 ng/mL in HEK-293 cell extract based on a 3.3 mg/mL extract load and 493.0 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP01412-2

  Cytometric bead array standard curve of MP01412-2, Cortactin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84569-2-PBS. Detection antibody: 84569-1-PBS. Standard: Ag1940. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84569-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

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Geprüfte Anwendungen

Produktinformation

84569-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Cortactin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cortactin fusion protein Ag1940
• Vollständiger Name: cortactin
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC008799
• Gene symbol: Cortactin
• Gene ID (NCBI): 2017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).