Cryptochrome 1 Polyklonaler Antikörper

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Cryptochrome 1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13474-1-PBS

Synonyme

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  WB analysis using 13474-1-AP (same clone as 13474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13474-1-AP (same clone as 13474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13474-1-AP (same clone as 13474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13474-1-AP (same clone as 13474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody (13474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13474-1-AP (same clone as 13474-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13474-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Cryptochrome 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cryptochrome 1 fusion protein Ag4270
• Vollständiger Name: cryptochrome 1 (photolyase-like)
• Berechnetes Molekulargewicht: 586 aa, 66 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC030519
• Gene symbol: CRY1
• Gene ID (NCBI): 1407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.