CstF-64 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CstF-64 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 26825-1-AP

Synonyme

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 26825-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26825-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 26825-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26825-1-AP

  IP Result of anti-CstF-64 (IP:26825-1-AP, 4ug; Detection:26825-1-AP 1:300) with HeLa cells lysate 2900ug.

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  IHC staining of human breast cancer using 26825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26825-1-AP (CstF-64 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26825-1-AP (CstF-64 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26825-1-AP (CstF-64 antibody) at dilution of 1:150 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Maushirngewebe, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

26825-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CstF-64 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CstF-64 fusion protein Ag25254
• Vollständiger Name: cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC017712
• Gene symbol: CSTF2
• Gene ID (NCBI): 1478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Publikationen

Species	Application	Title
human	CoIP	Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
human	IF	Biochem Biophys Res Commun; Cleavage stimulating factor 64 depletion mitigates cardiac fibrosis through alternative polyadenylation.; Authors - Rahul Neupane ; KD Validated
mouse	IHC	Front Oncol; CSTF2 Promotes Hepatocarcinogenesis and Hepatocellular Carcinoma Progression via Aerobic Glycolysis; Authors - Zhimin Chen
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
human	WB	J Hepatocell Carcinoma; A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma; Authors - Yangjin Huang