Cxcl2 Rekombinanter Antikörper

Cxcl2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240367C5

Kat-Nr. : 83758-1-RR

Synonyme

240367C5, CINC-3, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2

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  WB analysis of NR8383 using 83758-1-RR

  LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83758-1-RR (Cxcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 83758-1-RR

  Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using Cxcl2 antibody (83758-1-RR, Clone: 240367C5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83758-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83758-1-RR against Mouse Cxcl2 were performed. The affinity constant is 0.517 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LPS and Brefeldin A treated NR8383 cells
• Erfolgreiche Detektion in IF/ICC: PMA, LPS and Brefeldin A treated THP-1 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83758-1-RR bindet in WB, IF/ICC, ELISA Cxcl2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: chemokine (C-X-C motif) ligand 2
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: NM_053647.1
• Gene symbol: Cxcl2
• Gene ID (NCBI): 114105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).