Cyclin A1 Rekombinanter Antikörper

Cyclin A1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250550D7

Kat-Nr. : 86151-1-RR

Synonyme

CCNA1, Cyclin A, Cyclin-A1

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  WB analysis of SKOV-3 using 86151-1-RR

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 86151-1-RR (CCNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86151-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86151-1-RR (CCNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86151-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86151-1-RR against Human Cyclin A1 were performed. The affinity constant is 0.397 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, humanes Hodengewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86151-1-RR bindet in WB, ELISA Cyclin A1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cyclin A1 fusion protein Ag4005
• Vollständiger Name: cyclin A1
• Berechnetes Molekulargewicht: 464 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC036346
• Gene symbol: Cyclin A1
• Gene ID (NCBI): 8900
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNA1 binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. CCNA1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. In general, the expression of CCNA1 is tissue-specific and high CCNA1 expression is limited to testis; besides, lower levels of CCNA1 expression are also observed in other human cell lines and in healthy brain. Currently, CCNA1 expression has been illustrated to be downregulated in various tumors, including head and neck squamous-cell cancer (HNSCC) and nasopharyngeal carcinoma; and the promoter of the CCNA1 gene is found to be frequently methylated in colon cancer and HNSCC.