Cyclin A2 Monoklonaler Antikörper

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• KD/KO Validated

Cyclin A2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6C11

Kat-Nr. : 66391-1-PBS

Synonyme

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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  WB analysis using 66391-1-Ig (same clone as 66391-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

  WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66391-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Cyclin A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin A2 fusion protein Ag12765
• Vollständiger Name: cyclin A2
• Berechnetes Molekulargewicht: 432 aa, 49 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC104783
• Gene symbol: Cyclin A2
• Gene ID (NCBI): 890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.