Cyclin A2 Rekombinanter Antikörper

Cyclin A2 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C23

Kat-Nr. : 82148-1-PBS

Synonyme

CCNA2, Cyclin-A, Cyclin A, CCNA, CCN1

---

• 

  WB analysis using 82148-1-RR (same clone as 82148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82148-1-RR (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

  IP result of anti-Cyclin A2 (IP:82148-1-RR, 4ug; Detection:82148-1-RR 1:3000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 82148-1-RR (same clone as 82148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 82148-1-RR (same clone as 82148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82148-1-RR (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82148-1-RR (same clone as 82148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin A2 antibody (82148-1-RR, Clone: 1C23 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82148-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82148-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82148-1-RR against Human Cyclin A2 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

82148-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Cyclin A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cyclin A2 fusion protein Ag12765
• Vollständiger Name: cyclin A2
• Berechnetes Molekulargewicht: 432 aa, 49 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC104783
• Gene symbol: Cyclin A2
• Gene ID (NCBI): 890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.