Cyclin B1 Polyklonaler Antikörper

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• KD/KO Validated

Cyclin B1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55004-1-PBS

Synonyme

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

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  WB analysis using 55004-1-AP (same clone as 55004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55004-1-AP (same clone as 55004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55004-1-AP (same clone as 55004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 55004-1-AP (same clone as 55004-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 55004-1-AP (same clone as 55004-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55004-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, Indirect ELISA Cyclin B1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cyclin B1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: NM_031966
• Gene symbol: Cyclin B1
• Gene ID (NCBI): 891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.