Cyclin B2 Monoklonaler Antikörper

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• KD/KO Validated

Cyclin B2 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G8B7

Kat-Nr. : 67726-1-PBS

Synonyme

CCNB2, G2/mitotic-specific cyclin-B2, G2/mitotic specific cyclin B2, Cyclin B, 2G8B7

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  WB analysis using 67726-1-Ig (same clone as 67726-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67726-1-Ig (CCNB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67726-1-Ig (same clone as 67726-1-PBS)

  WB result of Cyclin B2 antibody (67726-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B2 transfected HeLa cells. This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67726-1-Ig (same clone as 67726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67726-1-Ig (Cyclin B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67726-1-Ig (same clone as 67726-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 67726-1-Ig (same clone as 67726-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Cyclin B2 antibody (67726-1-Ig, Clone: 2G8B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67726-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67726-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Cyclin B2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin B2 fusion protein Ag16329
• Vollständiger Name: cyclin B2
• Berechnetes Molekulargewicht: 398 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC105086
• Gene symbol: Cyclin B2
• Gene ID (NCBI): 9133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.