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Cyclin D1 Monoklonaler Antikörper
Cyclin D1 Monoklonal Antikörper für WB, FC (Intra)
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, FC (Intra)
Konjugation
Cardinal Red™ Fluorescent Dye
CloneNo.
2G3G5
Kat-Nr. : CR-60186
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | U2OS-Zellen, HeLa-Zellen, NIH/3T3-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:2000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CR-60186 bindet in WB, FC (Intra) Cyclin D1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Cyclin D1 fusion protein Ag0689 |
Vollständiger Name | cyclin D1 |
Berechnetes Molekulargewicht | 295 aa, 34 kDa |
Beobachtetes Molekulargewicht | 34 kDa |
GenBank-Zugangsnummer | BC000076 |
Gene symbol | Cyclin D1 |
Gene ID (NCBI) | 595 |
Konjugation | Cardinal Red™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 592 nm / 611 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Cardinal Red™ Cyclin D1 antibody CR-60186 | Protokoll herunterladen |
FC protocol for Cardinal Red™ Cyclin D1 antibody CR-60186 | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |