Cyclin E1 Rekombinanter Antikörper

Cyclin E1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240136D4

Kat-Nr. : 82700-10-RR

Synonyme

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

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  WB analysis of HT-29 using 82700-10-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82700-10-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 82700-10-RR

  rat heart tissue were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82700-10-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 82700-10-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 82700-10-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82700-10-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-10-RR against Human Cyclin E were performed. The affinity constant is 0.564 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HuH-7-Zellen, MCF-7-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82700-10-RR bindet in WB, FC (Intra), ELISA Cyclin E1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cyclin E1 fusion protein Ag2110
• Vollständiger Name: cyclin E1
• Berechnetes Molekulargewicht: 410 aa, 47 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC035498
• Gene symbol: Cyclin E1
• Gene ID (NCBI): 898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).