Cyclin E1 Rekombinanter Antikörper

Cyclin E1 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240136G9

Kat-Nr. : 82700-8-PBS

Synonyme

CCNE1, 240136G9, CCNE, Cyclin E, G1/S specific cyclin E1

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  Sandwich ELISA standard curve of MP00361-2

  Sandwich ELISA standard curve of MP00361-2, Human Cyclin E1 Monoclonal Matched Antibody Pair - PBS only. 82700-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2110. 82700-8-PBS was HRP conjugated as the detection antibody. Range: 0.313-40 ng/mL.

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  Sample test of MP00361-2

  The mean human Cyclin E1 concentration was determined to be 132.4 ng/mL in Hela cell extract based on a 3.0 mg/mL extract load.

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  IF Staining of HepG2 using 82700-8-RR (same clone as 82700-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82700-8-RR (same clone as 82700-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E1 antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82700-8-RR (same clone as 82700-8-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82700-8-RR (same clone as 82700-8-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00361-2

  Cytometric bead array standard curve of MP00361-2, Cyclin E1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-6-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 82700-8-PBS

  Biolayer interferometry (BLl) kinetic assay of 82700-8-PBS against Human Cyclin E1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 82700-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-8-RR against Human Cyclin E1 were performed. The affinity constant is 0.229 nM.

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Geprüfte Anwendungen

Produktinformation

82700-8-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Cyclin E1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cyclin E1 fusion protein Ag2110
• Vollständiger Name: cyclin E1
• Berechnetes Molekulargewicht: 410 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC035498
• Gene symbol: Cyclin E1
• Gene ID (NCBI): 898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).