Cyclin H Monoklonaler Antikörper

Cyclin H Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C5G11

Kat-Nr. : 67065-1-PBS

Synonyme

CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein

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  WB analysis using 67065-1-Ig (same clone as 67065-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67065-1-Ig (same clone as 67065-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67065-1-Ig (same clone as 67065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67065-1-Ig (same clone as 67065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 67065-1-Ig (same clone as 67065-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Cyclin H antibody (67065-1-Ig, Clone: 2C5G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 67065-1-Ig (same clone as 67065-1-PBS)

  1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin H (67065-1-Ig, Clone:2C5G11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67065-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Cyclin H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin H fusion protein Ag28556
• Vollständiger Name: cyclin H
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC005280
• Gene symbol: Cyclin H
• Gene ID (NCBI): 902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.