Cyclin T1 Rekombinanter Antikörper

Cyclin T1 Rekombinant Antikörper für WB, FC (Intra), ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

242256C3

Kat-Nr. : 84980-1-RR

Synonyme

CCNT1, 242256C3, CCNT, Cyclin T, Cyclin-T

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  WB analysis using 84980-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84980-1-RR (Cyclin T1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 84980-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of U2OS using 84980-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of HCT 116 using 84980-1-RR

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Cyclin T1 (84980-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84980-1-RR bindet in WB, FC (Intra), ELISA, ChIP-qPCR Cyclin T1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cyclin T1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 75-81 kDa
• GenBank-Zugangsnummer: NM_001240
• Gene symbol: Cyclin T1
• Gene ID (NCBI): 904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.