Cystatin B Polyklonaler Antikörper

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• KD/KO Validated

Cystatin B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10823-1-PBS

Synonyme

CSTB, EPM1, Cystatin-B, CST6, CPI-B

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  WB analysis of human saliva using 10823-1-AP (same clone as 10823-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10823-1-AP (same clone as 10823-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

  IP result of anti-Cystatin B (IP:10823-1-AP, 4ug; Detection:10823-1-AP 1:4000) with MCF-7 cells lysate 1320ug. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10823-1-AP (same clone as 10823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 10823-1-AP (same clone as 10823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10823-1-AP (same clone as 10823-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10823-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Cystatin B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cystatin B fusion protein Ag1262
• Vollständiger Name: cystatin B (stefin B)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC003370
• Gene symbol: Cystatin B
• Gene ID (NCBI): 1476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.