Cystatin C Rekombinanter Antikörper

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• KD/KO Validated

Cystatin C Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1I20

Kat-Nr. : 82441-1-PBS

Synonyme

CST3, 1I20, Gamma-trace, Cystatin-C, Cystatin-3

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  WB analysis using 82441-1-RR (same clone as 82441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82441-1-RR (Cystatin C antibody) at dilution of 1:3300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 82441-1-RR (same clone as 82441-1-PBS)

  WB result of Cystatin C antibody (82441-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells. This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 82441-1-RR (same clone as 82441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 82441-1-RR (same clone as 82441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82441-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82441-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82441-1-RR against Human Cystatin C were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82441-1-PBS bindet in WB, IHC, Indirect ELISA Cystatin C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cystatin C fusion protein Ag2890
• Vollständiger Name: cystatin C
• Berechnetes Molekulargewicht: 146 aa, 16 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC013083
• Gene symbol: Cystatin C
• Gene ID (NCBI): 1471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.