Cystatin S Polyklonaler Antikörper

Cystatin S Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 26979-1-AP

Synonyme

CST4, Cystatin 4, cystatin S, Cystatin SA III, Salivary acidic protein 1

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  WB analysis of human saliva using 26979-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 26979-1-AP (Cystatin S antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 26979-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26979-1-AP (Cystatin S antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26979-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26979-1-AP (Cystatin S antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Speichelgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

26979-1-AP bindet in WB, IHC, ELISA Cystatin S und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cystatin S fusion protein Ag25619
• Vollständiger Name: cystatin S
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC065714
• Gene symbol: Cystatin S
• Gene ID (NCBI): 1472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatins are a family of inhibitors of cysteine peptidases that comprises the salivary cystatins D and S-type (including CST5 and CST4,1,2) and cystatin C-type (CST3) (PMID: 25329717). The D and S-type cystatin have also been detected in seminal plasma, tears, and tracheobronchial fluid but not in other fluids and secretions where cystatin C is found. And the CST4 was expressed in the serous acinar and demilune cells of the human submandibular gland and at lower levels in the serous acini of the parotid gland (PMID:11879580). CST4 specifically combines with cysteine protease to regulate its activity, thus preventing hydrolysis of the extracellular matrix. And some studies propose that CST4 might be a biomarker for gastrointestinal cancer (PMID:29218251; PMID:29636621).

Publikationen

Species	Application	Title
human	IHC	Life Sci; Prognostic value of immune-related cells and genes in the tumor microenvironment of ovarian cancer, especially CST4.; Authors - Shuang Wang