Cystatin SN/CST1 Rekombinanter Antikörper

Cystatin SN/CST1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242888B3

Kat-Nr. : 85403-1-RR

Synonyme

CST1, Cystain SA I, Cystain-SA-I, Cystatin 1, cystatin SN

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  WB analysis using 85403-1-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 85403-1-RR (CST1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 85403-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 85403-1-RR (Cystatin SN/CST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85403-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85403-1-RR against Human Cystatin SN/CST1 were performed. The affinity constant is 1.09 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, human saliva

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85403-1-RR bindet in WB, ELISA Cystatin SN/CST1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cystatin SN/CST1 fusion protein Ag8862
• Vollständiger Name: cystatin SN
• Berechnetes Molekulargewicht: 141 aa, 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa
• GenBank-Zugangsnummer: BC021225
• Gene symbol: CST1
• Gene ID (NCBI): 1469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.