Cytochrome P450 Reductase Polyklonaler Antikörper

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Cytochrome P450 Reductase Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 29814-1-AP

Synonyme

POR, P450R, CYPOR, CPR

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  WB analysis using 29814-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 29814-1-AP

  WB result of Cytochrome P450 Reductase antibody (29814-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome P450 Reductase transfected HepG2 cells.

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  WB analysis using 29814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29814-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 29814-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29814-1-AP bindet in WB, IHC, ELISA Cytochrome P450 Reductase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytochrome P450 Reductase fusion protein Ag30899
• Vollständiger Name: P450 (cytochrome) oxidoreductase
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: NM_000941
• Gene symbol: Cytochrome P450 Reductase
• Gene ID (NCBI): 5447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa.

Publikationen

Species	Application	Title
human	WB	iScience; AMPD3 promotes doxorubicin-induced cardiomyopathy through HSP90α-mediated ferroptosis; Authors - Liting Cheng