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Cytochrome c Monoklonaler Antikörper

Cytochrome c Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(138)

CloneNo.

2D8D11

Kat-Nr. : 66264-1-Ig

Synonyme

CYCS, CYC, 2D8D11



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, MCF-7-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe, RAW 264.7-Zellen, ROS1728-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:1000-1:5000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66264-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Cytochrome c und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hund, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen Cytochrome c fusion protein Ag24349
Vollständiger Name cytochrome c, somatic
Berechnetes Molekulargewicht 12 kDa
Beobachtetes Molekulargewicht 12-15 kDa
GenBank-ZugangsnummerBC009578
Gene symbol Cytochrome c
Gene ID (NCBI) 54205
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Cytochrome c antibody 66264-1-IgProtokoll herunterladen
IHC protocol for Cytochrome c antibody 66264-1-IgProtokoll herunterladenl
IF protocol for Cytochrome c antibody 66264-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB

Hepatology

Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation.

Authors - Chao Zhang
human

Cell Death Differ

ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity

Authors - Feng Xu
humanIF

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
humanWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
humanWB

ACS Appl Mater Interfaces

Extracellular Matrix-Mimicking Hydrogel with Angiogenic and Immunomodulatory Properties Accelerates Healing of Diabetic Wounds by Promoting Autophagy

Authors - Yujie Pan
humanIF

J. Pineal Res.

Human transporters, PEPT1/2, facilitate melatonin transportation into mitochondria of cancer cells: an implication of the therapeutic potential.

Authors - Xiaokui Huo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Megha (Verified Customer) (07-10-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Capan-1 cells