Cytochrome c Rekombinanter Antikörper

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Cytochrome c Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230503E10

Kat-Nr. : 83276-1-PBS

Synonyme

230503E10, CYC, CYCS

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  WB analysis using 83276-1-RR (same clone as 83276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83276-1-RR (same clone as 83276-1-PBS)

  WB result of Cytochrome c antibody (83276-1-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83276-1-RR (same clone as 83276-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cytochrome c antibody (83276-1-RR, Clone: 230503E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83276-1-RR (same clone as 83276-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83276-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA Cytochrome c und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytochrome c fusion protein Ag1455
• Vollständiger Name: cytochrome c, somatic
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12-15 kDa
• GenBank-Zugangsnummer: BC009578
• Gene symbol: Cytochrome c
• Gene ID (NCBI): 54205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.