Cytokeratin 10 Polyklonaler Antikörper

Cytokeratin 10 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18343-1-PBS

Synonyme

KRT10, K10, Cytokeratin-10, Cytokeratin,Keratin, CK-10

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  WB analysis using 18343-1-AP (same clone as 18343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 18343-1-AP (same clone as 18343-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 18343-1-PBS

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 18343-1-PBS

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 18343-1-AP (same clone as 18343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 18343-1-AP (same clone as 18343-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 18343-1-AP (same clone as 18343-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 18343-1-AP (same clone as 18343-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 10 (18343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18343-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18343-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Cytokeratin 10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 10 fusion protein Ag13136
• Vollständiger Name: keratin 10
• Berechnetes Molekulargewicht: 584 aa, 59 kDa
• Beobachtetes Molekulargewicht: 50-59 kDa
• GenBank-Zugangsnummer: BC034697
• Gene symbol: Cytokeratin 10
• Gene ID (NCBI): 3858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.