Cytokeratin 10 Rekombinanter Antikörper

Cytokeratin 10 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

250893H2

Kat-Nr. : 86324-1-RR

Synonyme

KRT10, CK 10, CK10, CK-10, Cytokeratin,Keratin

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  WB analysis using 86324-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86324-1-RR (KRT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 86324-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 86324-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 86324-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 86324-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86324-1-RR bindet in WB, IHC, IF-P, ELISA Cytokeratin 10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 10
• Berechnetes Molekulargewicht: 584 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC034697
• Gene symbol: Cytokeratin 10
• Gene ID (NCBI): 3858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 10 (CK-10, K10, KRT10) is a type I (acidic) cytokeratin and part of the intermediate filament protein superfamily. It is expressed in keratinized stratified epithelium, suprabasal cell layers (including the stratum corneum), and differentiated areas of highly differentiated squamous cell carcinomas. CK-10 plays a role in maintaining the structural integrity of epithelial cells and is involved in differentiation, tissue specialization, and epidermal barrier establishment. Mutations in the KRT10 gene are associated with epidermolytic hyperkeratosis, and its expression is linked to the terminal differentiation of epidermal cells . CK-10 is also used as a marker for tissue differentiation and characterization of malignant tumors in surgical pathology.