Cytokeratin 14 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 14 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite®488 Fluorescent Dye

CloneNo.

2G1E2

Kat-Nr. : CL488-60320

Synonyme

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

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  IF Staining of mouse skin using CL488-60320

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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  IF Staining of mouse skin using CL488-60320

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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  IF Staining of A431 using CL488-60320

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CL488-60320 (Cytokeratin 14 antibody) at dilution of 1:100.

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  IF Staining of A431 using CL488-60320

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-60320

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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  IF Staining of HaCaT using CL488-60320

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushautgewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HaCaT-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60320 bindet in IF/ICC, IF-P Cytokeratin 14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Vollständiger Name: keratin 14
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC002690
• Gene symbol: Cytokeratin 14
• Gene ID (NCBI): 3861
• Konjugation: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.