Cytokeratin 16 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 16 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2H4D8

Kat-Nr. : 66802-1-Ig

Synonyme

KRT16, 2H4D8, CK 16, CK16, CK-16

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  WB analysis of A431 using 66802-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66802-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human milk using 66802-1-Ig

  human milk were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 66802-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (66802-1-Ig, Clone: 2H4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Zervixkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

66802-1-Ig bindet in WB, IHC, IF/ICC, ELISA Cytokeratin 16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 16 fusion protein Ag11240
• Vollständiger Name: keratin 16
• Berechnetes Molekulargewicht: 473 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC039169
• Gene symbol: Cytokeratin 16
• Gene ID (NCBI): 3868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Cancers (Basel); Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.; Authors - Maha Elazezy ; KD Validated
human	WB,IHC	Nat Commun; Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer; Authors - Xuejiao Liu
human	WB,IHC	Genomics; Identification of prognostic biomarkers for cholangiocarcinoma by combined analysis of molecular characteristics of clinical MVI subtypes and molecular subtypes; Authors - Ming-Yue Li ; KD Validated
human	WB	J Photochem Photobiol B; Acne-related UVA-induced facial fluorescence: An exploratory study from physiological properties to tissue structure information; Authors - Xing Ren