Cytokeratin 18 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Cytokeratin 18 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F2

Kat-Nr. : 82817-5-PBS

Synonyme

KRT18, 4F2, Cell proliferation-inducing gene 46 protein, CK 18, CK18

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  WB analysis of HeLa using 82817-5-RR (same clone as 82817-5-PBS)

  WB result of Cytokeratin 18 antibody (82817-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 18 transfected HeLa cells. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (82817-5-RR, Clone: 4F2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82817-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Cytokeratin 18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.