Cytokeratin 18 Monoklonaler Antikörper

Cytokeratin 18 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®568 Fluorescent Dye

CloneNo.

1G11C4

Kat-Nr. : CL568-66187

Synonyme

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie der Validierungsdaten

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  IF Staining of HUVEC using CL568-66187

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®568 Cytokeratin 18 antibody (CL568-66187, Clone: 1G11C4 ) at dilution of 1:800.

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  IF Staining of HUVEC using CL568-66187

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®568 Cytokeratin 18 antibody (CL568-66187, Clone: 1G11C4 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HUVEC-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL568-66187 bindet in IF Cytokeratin 18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: KRT18
• Gene ID (NCBI): 3875
• Konjugation: CoraLite®568 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 568 nm / 587 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.