Cytokeratin 19 Polyklonaler Antikörper

Cytokeratin 19 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL594-10712

Synonyme

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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  IF Staining of HeLa using CL594-10712

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cytokeratin 19 antibody (CL594-10712) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-10712

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Cytokeratin 19 antibody (CL594-10712) at dilution of 1:200.

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  FC experiment of MCF-7 using CL594-10712

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Cytokeratin 19 (CL594-10712) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-10712 bindet in IF/ICC, FC (Intra) Cytokeratin 19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag1085
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC007628
• Gene symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Publikationen

Species	Application	Title
human	IF	Biosens Bioelectron; Constructing a highly sensitive duplex immunoassay using AuNPs and AgNPs as nanolabels for investigating the epithelial-mesenchymal transition occurring on circulating tumor cells with lung cancer patients; Authors - Yunuo Dai