Cytokeratin 20 Monoklonaler Antikörper

Cytokeratin 20 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4D10A4

Kat-Nr. : 60183-1-PBS

Synonyme

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

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  WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens) showing negative staining.. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:400 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Collagen Type III (N-terminal) antibody (22734-1-AP, green), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60183-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Cytokeratin 20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 20 fusion protein Ag11336
• Vollständiger Name: keratin 20
• Berechnetes Molekulargewicht: 424 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC031559
• Gene symbol: Cytokeratin 20
• Gene ID (NCBI): 54474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.