Cytokeratin 39 Rekombinanter Antikörper

Cytokeratin 39 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250891F5

Kat-Nr. : 86277-2-PBS

Synonyme

CK-39, Cytokeratin-39, K39, KA35, Keratin, type I cytoskeletal 39

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  WB analysis using 86277-2-RR (same clone as 86277-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  WB analysis using 86277-2-RR (same clone as 86277-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86277-2-RR (same clone as 86277-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86277-2-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Cytokeratin 39 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 39
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC157887
• Gene symbol: KRT39
• Gene ID (NCBI): 390792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 39 (CK-39) is a member of the cytokeratin family, a group of intermediate filament proteins primarily expressed in epithelial cells. Cytokeratins are classified into Type I (acidic) and Type II (basic) subfamilies, which pair to form heteropolymers essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. CK-39 is predominantly expressed in stratified and glandular epithelia, such as those found in the prostate, bladder, and respiratory tract. Its expression pattern may vary depending on tissue differentiation states.