Cytokeratin 5/6 Monoklonaler Antikörper

Cytokeratin 5/6 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9E1

Kat-Nr. : 68295-1-PBS

Synonyme

KRT6B, 1F9E1, CK 6B, CK5/6, CK6B

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  WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68295-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Cytokeratin 5/6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 5/6 fusion protein Ag11288
• Vollständiger Name: keratin 6B
• Berechnetes Molekulargewicht: 564 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC034535
• Gene symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.