Cytokeratin 5 Polyklonaler Antikörper

Cytokeratin 5 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 28506-1-AP

Synonyme

CK 5, CK-5, keratin 5, KRT5, Cytokeratin5

---

• 

  WB analysis using 28506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 28506-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, mouse skin tissues, rat skin tissues
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 7 publications below

Produktinformation

28506-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA Cytokeratin 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 5 fusion protein Ag25436
• Vollständiger Name: keratin 5
• Berechnetes Molekulargewicht: 590 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC024292
• Gene symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells

Publikationen

Species	Application	Title
human	IF	Stem Cell Res Ther; Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2; Authors - Zhilin Sui
human	IF	J Invest Dermatol; Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo.; Authors - Li Dong
mouse	IF	FASEB J; Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia; Authors - Sheng Gao
human	WB	J Clin Lab Anal; Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis.; Authors - Shan Chen
mouse	IF	J Oral Biosci; Epigenetic modifier G9a is involved in regulation of mouse tongue development; Authors - Hisashi Ideno
human	IF	Cancer Lett; The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma; Authors - Zhe Yi Wang