Cytokeratin 5 Monoklonaler Antikörper

Cytokeratin 5 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1C5

Kat-Nr. : CL488-66727

Synonyme

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

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  IF Staining of human oesophagus cancer using CL488-66727

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 488 Cytokeratin 5 antibody (CL488-66727, Clone: 1A1C5 ) at dilution of 1:200.

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  IF Staining of human oesophagus cancer using CL488-66727

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 488 Cytokeratin 5 antibody (CL488-66727, Clone: 1A1C5 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Ösophaguskarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66727 bindet in IF-P Cytokeratin 5 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 5 fusion protein Ag24184
• Vollständiger Name: keratin 5
• Berechnetes Molekulargewicht: 590 aa, 62 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC024292
• Gene symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.