Cytokeratin 6A Monoklonaler Antikörper

Cytokeratin 6A Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2H6A11

Kat-Nr. : 66685-1-Ig

Synonyme

Cytokeratin 6, KRT6A, 2H6A11, CK 6A, CK6

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  WB analysis of A431 using 66685-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66685-1-Ig (KRT6A antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66685-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66685-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66685-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66685-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

66685-1-Ig bindet in WB, IHC, IF, ELISA Cytokeratin 6A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 6A fusion protein Ag0882
• Vollständiger Name: keratin 6A
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC008807
• Gene symbol: Cytokeratin 6A
• Gene ID (NCBI): 3853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A, 6B and 6C. They share more than 99% identical DNA sequence.

Publikationen

Species	Application	Title
human	IHC,IF	Aging (Albany NY); Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma.; Authors - Junfeng Zhang
mouse	IF	Cell Tissue Res; Sodium alginate/collagen hydrogel loaded with human umbilical cord mesenchymal stem cells promotes wound healing and skin remodeling.; Authors - Zhenkun Zhang
human	WB	Viruses; KRT6A Restricts Influenza A Virus Replication by Inhibiting the Nuclear Import and Assembly of Viral Ribonucleoprotein Complex; Authors - Yu Chang ; KD Validated