Cytokeratin 6B Polyklonaler Antikörper

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• KD/KO Validated

Cytokeratin 6B Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17391-1-PBS

Synonyme

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  WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 17391-1-AP (same clone as 17391-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skin using 17391-1-AP (same clone as 17391-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17391-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Cytokeratin 6B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 6B fusion protein Ag11288
• Vollständiger Name: keratin 6B
• Berechnetes Molekulargewicht: 564 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC034535
• Gene symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.