Cytokeratin 6B Rekombinanter Antikörper

Cytokeratin 6B Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242874F9

Kat-Nr. : 85423-2-PBS

Synonyme

KRT6B, 242874F9, CK 6B, CK5/6, CK6B

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  WB analysis using 85423-2-RR (same clone as 85423-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85423-2-RR (KRT6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85423-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 85423-2-RR (same clone as 85423-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85423-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 85423-2-RR (same clone as 85423-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85423-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 85423-2-RR (same clone as 85423-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85423-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 85423-2-RR (same clone as 85423-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85423-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85423-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85423-2-RR against Human Cytokeratin 6B were performed. The affinity constant is 4.41 nM.

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Geprüfte Anwendungen

Produktinformation

85423-2-PBS bindet in WB, IHC, Indirect ELISA Cytokeratin 6B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytokeratin 6B fusion protein Ag11288
• Vollständiger Name: keratin 6B
• Berechnetes Molekulargewicht: 564 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC034535
• Gene symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 6B (CK6B) is a type of intermediate filament protein primarily expressed in certain epithelial cells. Cytokeratin 6B consists of a central α-helical rod domain flanked by non-helical head and tail domains, which is a common structural feature of cytokeratins. CK6B is mainly expressed in the basal and suprabasal layers of stratified epithelia, such as the oral mucosa, esophagus, and conjunctiva. It is also found in some specialized epithelial cells like the hair follicle outer root sheath and the nail matrix. CK6B can serve as a diagnostic marker for certain pathological conditions. Its expression pattern can be used to distinguish between different types of epithelial tumors or to identify specific epithelial cell populations in histopathological studies.