Cytokeratin 6B Rekombinanter Antikörper

Cytokeratin 6B Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242874F9

Kat-Nr. : 85423-2-RR

Synonyme

KRT6B, 242874F9, CK 6B, CK5/6, CK6B

---

• 

  WB analysis using 85423-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85423-2-RR (KRT6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85423-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85423-2-RR against Human Cytokeratin 6B were performed. The affinity constant is 4.41 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HaCaT-Zellen, Maushautgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85423-2-RR bindet in WB, IHC, ELISA Cytokeratin 6B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytokeratin 6B fusion protein Ag11288
• Vollständiger Name: keratin 6B
• Berechnetes Molekulargewicht: 564 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC034535
• Gene symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 6B (CK6B) is a type of intermediate filament protein primarily expressed in certain epithelial cells. Cytokeratin 6B consists of a central α-helical rod domain flanked by non-helical head and tail domains, which is a common structural feature of cytokeratins. CK6B is mainly expressed in the basal and suprabasal layers of stratified epithelia, such as the oral mucosa, esophagus, and conjunctiva. It is also found in some specialized epithelial cells like the hair follicle outer root sheath and the nail matrix. CK6B can serve as a diagnostic marker for certain pathological conditions. Its expression pattern can be used to distinguish between different types of epithelial tumors or to identify specific epithelial cell populations in histopathological studies.