Cytokeratin 7 Polyklonaler Antikörper

Cytokeratin 7 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-15539

Synonyme

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL555-15539

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

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  IF Staining of HeLa using CL555-15539

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

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  IF Staining of human breast cancer using CL555-15539

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

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  IF Staining of human breast cancer using CL555-15539

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

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  FC experiment of HeLa using CL555-15539

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 7 (CL555-15539) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-15539 bindet in IF, FC (Intra) Cytokeratin 7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC002700
• Gene symbol: KRT7
• Gene ID (NCBI): 3855
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C