Cytokeratin 7 Polyklonaler Antikörper

Cytokeratin 7 Polyklonal Antikörper für IF/ICC, IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, IF-P, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-15539

Synonyme

KRT7, keratin 7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

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  IF Staining of human breast cancer using CL555-15539

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

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  IF Staining of human breast cancer using CL555-15539

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

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  IF Staining of HepG2 using CL555-15539

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

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  IF Staining of HeLa using CL555-15539

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

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  FC experiment of HeLa using CL555-15539

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated Cytokeratin 7 Polyclonal antibody (CL555-15539)(red), or 0.8 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-15539 bindet in IF/ICC, IF-P, FC (Intra) Cytokeratin 7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC002700
• Gene symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.