Cytokeratin 79 Rekombinanter Antikörper

Cytokeratin 79 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250927D8

Kat-Nr. : 86310-1-PBS

Synonyme

CK-79, Cytokeratin-79, K6L, K79, KB38

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  WB analysis of mouse skin using 86310-1-RR (same clone as 86310-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 86310-1-RR (KRT79 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 86310-1-RR (same clone as 86310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 86310-1-RR (same clone as 86310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 86310-1-RR (same clone as 86310-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skin using 86310-1-RR (same clone as 86310-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 79 antibody (86310-1-RR, Clone: 250927D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86310-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86310-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Cytokeratin 79 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 79
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC039148
• Gene symbol: KRT79
• Gene ID (NCBI): 338785
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KRT79(Keratin 79, also known as K6L or KRT6L) is a type II epithelial keratin, which is mainly expressed in skeletal muscle, skin, scalp and other tissues and participates in maintaining the structural integrity of epithelial cells. KRT79 plays an important role in hair follicle development, and it is one of the marker genes of hair follicle outer root sheath and hair stromal cells (HG cells). Together with KRT17 and SOX9, it marks HG cells and participates in hair follicle regeneration and morphogenesis.